Composite

Part:BBa_I723016:Design

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)


DntR transcriptional regulator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 339
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 666
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 957
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 568


Design Notes

The precise boundaries of the promoters and ribosome binding sites are unknown, however they are known to exist back-to-back and were consequently cloned as one entire physical unit.

The transcriptional regulator is therefore in the opposite orientation to its target promoter.

Source

Cloned from a historical plasmid based on pQF52 carrying genomic sequences from Burkholderia cepacia.

References